Bringing Your Genetics to Life

Embryo Transfer

Embryo transfer is a procedure where an embryo is collected (flushed) from one female (donor) and then transferred to the uterus of another female (the recipient) for the duration of gestation.

On the farm with mobile lab

In-Vitro Fertilization

Procedure

The process is considered non-surgical and can be completed in less than 20 minutes per cow. Only an epidural is used for anesthesia. A transvaginal ultrasound probe is inserted in the vagina where it is used to guide a 17gauge, 22-inch needle into the follicles. At that point a vacuum pump is used to aspirate the oocytes. The needle is carefully guided from follicle to follicle gently removing the oocytes from each one. These oocytes are then taken to our laboratory and allowed to mature in a controlled environment for 24 hours before the fertilization process begins.

InVitro Fertilization

Frozen, Fresh or sexed semen can be used. The quality of the semen is just as important as the oocytes and must be evaluated prior to use. A set of control oocytes from abattoir ovaries is used to determine the optimum fertilization rates. This time consuming procedure is essential to the process and will result in the highest fertilization rates in vitro.

Culture

The next 6 days following the fertilization are very important in the young embryos life. Precise laboratory conditions are essential and are closely watched. Everything possible is done to duplicate the chemical conditions that would be in the cow's reproductive tract. While the embryos are growing in the laboratory the recipients are being synchronized in the field.

Candidates
  • Older Cows ̶ extends reproductive life
  • Problem Cows ̶ due mainly to fertilization failure when super ovulated
  • Healthy Cycling Cows ̶ Donor cows enrolled in conventional flush program can also be producing embryos via IVF giving more options on sires
  • Pregnant Cows and Heifers ̶ During first trimester embryos can be produced without affecting the developing fetus.
  • Prepuberal Heifers ̶ viable oocytes can be produced even before estrus cycles begin
  • Postpartum Cows ̶ days after calves and prior to normal estrus cycles
Definitions:
  • Donor: The female from which oocytes or embryos are recovered
  • Embryo: a fertilized egg
  • Fetus: The phase of development following the embryo stage
  • Follicle: a structure on the ovary, which contains the immature ovum
  • In vitro: Latin for in glass; observable in a test tube; in an artificial environment
  • In vivo: Latin for within the living body
  • Oocytes: Unfertilized egg or ovum as harvested directly from the follicle
  • Ovulation: Natural release of an ovum from the follicle
  • Ovum: Latin for egg: usually indicates one unfertilized egg
  • Recipient: the female that will receive an embryo
  • Capacitation: The process by which spermatozoa become capable of fertilizing an ovum
"Only a few labs that have the capabilities of Ovagenix."

Embryo Export

OvaGenix is a USDA certified export center for movement of embryos into The European Union, Central America, South America and Mexico.
Embryos must be collected by OvaGenix and meet the requirements for each country. Please contact OvaGenix for more information.

Embryo Export Requirements

OvaGenix is certified to collect and export embryos to all countries including the European Continent.

All embryos collected and frozen by OvaGenix are treated under IETS guidelines for all countries.


Additional Information:
  • ALL Bulls used for exportable breedings MUST be CSS certified and owner must provide certificate.
  • Health testing requirements vary greatly between importing countries. OvaGenix will provide specific requirements upon request.
  • In general, Certified TB and Brucellosis Free herds will qualify for export to most countries.

New Client Information

Services Provided
  • Conventional Embryo Transfer
    • Donor Superovulation and Collection
    • Donor Single Embryo Collection
    • Embryo Freezing and Storage
    • Embryo Transfer
    • On the Farm Collections
    • Embryo Shipping
  • Special Services
    • In-Vitro Fertilization (IVF)
    • Ultra Sound Diagnostics
    • Fetal Sexing
    • Tissue Banking

OvaMex

OvaGenix's work in Mexico was first established in 2004 and has expanded thanks to our Texas breeders who have sold embryos to our Mexican clients. Our legal Mexican company can assist with importation of semen, embryos and equipment from the USA and provide documentation and safe delivery to the Mexican client's ranches. The OvaMex team prides themselves on the highest results. Through the work of our OvaMex veterinarians who travel to the ranches to evaluate, train and verify all the procedures we prescribe for donors and recipients, we are able to provide successful outcomes for our clients. We have worked in all areas of Mexico; tropical, high desert and coastal.

Services:
  • Males: Evaluation of fertility, semen collection and processing including cryopreservation with inventory/storage and shipping to end users.
  • Females: Evaluation of fertility, embryo and oocyte collection and processing including transferring to recipients either from cryopreservation or fresh collection.
Products:
  • Embryo Transfer supplies, estrus synchronization drugs and detection devices.
  • All products dealing with reproduction and providing the service of reproductive technology.
  • Semen (sperm) from proven males (fresh and frozen; conventional or preferred sex).
  • Embryos from proven females inseminated with semen from proven males.
  • Embryos produced from collection of superovualted females (in vivo) or from in vitro (IVF) fertilization production.
  • Drugs and devices for estrus and ovulation control of females and super ovulation.
  • Equipment and supplies both disposable and small tools for semen and embryo production both in vivo and in vitro methods. Micromanipulation equipment and associated materials for microsurgery of embryos and sperm injection.
  • Nutritional supplements including injectable vitamins and minerals for livestock.
  • Specialized concentrated feed mixes with balanced nutrition for both ruminants and monograstiric animals.
Consultation:
  • Laboratory design for semen and embryo (conventional and in vitro) production.
  • Evaluate donor bulls and cows for impaired fertility and devise methods to circumvent the problems and provide guidance for restoring fertility.
  • Inventory of liquid nitrogen vessels storing frozen semen and embryos.